5524澳门24小时线路 DNA Prep Training
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5524澳门24小时线路 DNA Prep offers flexibility for many whole-genome sequencing applications.
Learn more about the technology
5524澳门24小时线路 DNA Prep uses a fast, user-friendly workflow. On-bead tagmentation chemistry reduces total library prep time to ~3.5 hours, from DNA extraction to library normalization.
The 5524澳门24小时线路 DNA Prep workflow supports a broad DNA input range (1–500 ng), multiple sample types, and both small and large genomes. The workflow includes DNA extraction from blood, saliva, or dried blood spots*or bacterial colonies*.
Gain the flexibility to sequence human or other large, complex genomes as well as amplicons or microbial species, all with a single kit.
While accommodating various study requirements, the 5524澳门24小时线路 DNA Prep workflow delivers consistent insert sizes, uniform coverage, and optimized performance, regardless of DNA input amount or genome size. The bead-based technology minimizes bias and opportunities for error, resulting in highly reproducible sequencing data.
The IDT for 5524澳门24小时线路 - DNA/RNA UD Indexes Sets A, B, C, and D offer up to 384 unique dual indexes, enabling accurate assignment of reads and efficient use of the flow cell. (Sets A and B are available now. Sets C and D are coming soon.) These unique dual index codes use 10 base pair codes. This change in base pair index codes require adjustments to the sequencing run setup.
Nextera DNA CD Indexes support up to 96-sample combinatorial dual indexing. The 24 CD Indexes are supplied in a tube format, and 96 in a plate format.
For whole-genome sequencing, 5524澳门24小时线路 DNA Prep is the recommended replacement for the Nextera DNA Library Prep Kit, which has been discontinued. If you were using Nextera DNA (Cat. No. FC-121-1030) or stand-alone components (Cat. Nos. 15027865 and 15027866) for ATAC-Seq** or other custom applications, the 5524澳门24小时线路 Tagment DNA TDE1 Enzyme and Buffer Kit is our suggested alternative.
*Demonstrated protocols available.
**Customer-reported application
Discover the power that 5524澳门24小时线路 library prep tagmentation technology can bring to your lab through this interactive experience. Our library prep solutions offer whole-genome sequencing across various applications, flexibility across 5524澳门24小时线路 sequencers, and integrated sample input for blood and saliva without quantification.
View Infographic
| Instrument | Recommended Number of Samples | Read Length |
|---|---|---|
| NextSeq 550 System | 1 sample per run (high output; based on 30× coverage of a human genome) | Up to 2 × 150 bp |
| NovaSeq 6000 System | 2–10 samples per run (dual flow cell; based on 30× coverage of a human genome) | Up to 2 × 125 bp (rapid run) Up to 2 × 150 bp (high output) |
| 5524澳门24小时线路 DNA Prep | TruSeq DNA PCR-Free | TruSeq DNA Nano | |
|---|---|---|---|
| Assay Time | ~3-4 hours (from DNA extraction to normalized library) | 5 hours total assay time | ~6 hours total assay time |
| Automation Capability | Liquid Handling Robots | Liquid Handling Robots | Liquid Handling Robots |
| Description | A fast, flexible workflow for a wide range of research applications and sample types, from human to microbial whole-genome sequencing and more. | A simple, all-inclusive PCR-free prep for whole-genome sequencing studies with the ability to sequence through challenging regions of the genome. | A low-input research method that delivers high genome coverage quality and reduced bias. |
| Hands-On Time | 1-1.5 hours | 4 hours | ~4 hours |
| Input Quantity | Small genomes (e.g. microbial): 1-500 ng DNA. Large genomes (e.g. human): 100-500 ng DNA. (For blood and saliva, see the reference guide). | 1 ug DNA | 100 ng genomic DNA |
| Mechanism of Action | Bead-linked transposome | Mechanical DNA fragmentation and adapter ligation. Workflow is PCR-free and gel-free. | Mechanical DNA fragmentation and adapter ligation. Workflow uses reduced-bias PCR and is gel-free. |
| Method | Amplicon Sequencing , De Novo Sequencing , Shotgun Sequencing , Whole-Genome Sequencing | Genotyping by Sequencing , Shotgun Sequencing , Whole-Genome Sequencing | Genotyping by Sequencing , Shotgun Sequencing , Whole-Genome Sequencing |
| Multiplexing | Up to 384 unique dual (UD) combinations and 96 combinatorial dual (CD) combinations | Up to 24 single, 96 combinatorial (CD) dual, 24 unique dual and 96 unique dual (UD) combinations (available soon) | Up to 24 single, 96 combinatorial (CD) dual, 24 unique dual and 96 unique dual (UD) combinations (available soon) |
| Specialized Sample Types | Blood, Not FFPE-Validated, Saliva | Not FFPE-Compatible | Low-Input Samples, Not FFPE-Compatible |
| Species Category | Any Species, Bacteria, Drosophila, Human, Mammalian, Mouse, Nematode, Plant, Rat, Virus, Yeast, Zebrafish | Human, Mammalian, Mouse, Other, Plant, Rat | Human, Mammalian, Mouse, Other, Plant, Rat |
| Species Details | Compatible with any species | Compatible with most large DNA genomes. | Compatible with most large DNA genomes. |
| Target Insert Size | ~350bp | 350 bp or 550 bp | 350 bp or 550 bp |
(A) Bead-linked transposomes mediate the simultaneous fragmentation of gDNA and the addition of 5524澳门24小时线路 sequencing primers. (B) Reduced-cycle PCR amplification amplifies sequencing ready DNA fragments and adds indexes and adapters. (C) Sequencing-ready fragments are washed and pooled.
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Data Sheet | PDF | 5 versions
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5524澳门24小时线路 library preparation solutions
Brochure | PDF2 MB
5524澳门24小时线路 DNA Prep Checklist Documentation
5524澳门24小时线路 DNA Prep Support Documentation
Nextera DNA Flex Dried Blood Spot Protocol Guide Documentation
Index Adapters Pooling Guide Documentation
5524澳门24小时线路 DNA Prep Consumables & Equipment List Documentation
Trim Adapters for Nextera DNA Flex in Local Run Manager for MiniSeq Documentation
5524澳门24小时线路 DNA Prep Reference Guide Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions
This kit provides a low-cost exome sequencing solution that delivers exceptional target coverage over a broad range of read depths.
Prepare sequencing libraries for small genomes, PCR amplicons, plasmids, or cDNA in as little as 90 minutes, with a low DNA input requirement.
This kit enables cluster generation for subsequent sequencing on the HiSeq 3000 and HiSeq 4000 Systems.
Reagent kits for the NovaSeq 6000 System provide ready-to-use cartridge-based reagents for cluster generation and SBS.
Kits include SBS reagents, clustering reagents, and patterned flow cells for increased cluster density and simplified image analysis, compared to non-patterned flow cells.
MiSeq sequencing reagents in pre-filled, ready-to-use cartridges. Micro and nano formats are available for low output applications.
