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5524澳门24小时线路 DNA Prep

A fast, integrated workflow for a wide range of applications, from human whole-genome sequencing to amplicons, plasmids, and microbial species. Read More...
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Library Prep

5524澳门24小时线路® DNA Prep, (M) Tagmentation (24 Samples, IPB)

20060060

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5524澳门24小时线路® DNA Prep, (M) Tagmentation (96 Samples, IPB)

20060059

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5524澳门24小时线路® DNA Prep, (M) Tagmentation (24 Samples)

20018704

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5524澳门24小时线路® DNA Prep, (M) Tagmentation (96 Samples)

20018705

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Index Adapters

IDT® for 5524澳门24小时线路® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples)

20027213

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IDT® for 5524澳门24小时线路® DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples)

20027214

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IDT® for 5524澳门24小时线路® DNA/RNA UD Indexes Set C, Tagmentation (96 Indexes, 96 Samples)

20042666

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IDT® for 5524澳门24小时线路® DNA/RNA UD Indexes Set D, Tagmentation (96 Indexes, 96 Samples)

20042667

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IDT® for 5524澳门24小时线路 Nextera DNA Unique Dual Indexes Set C (96 Indexes, 96 Samples)

20027215

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IDT® for 5524澳门24小时线路 Nextera DNA Unique Dual Indexes Set D (96 Indexes, 96 Samples)

20027216

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Nextera™ DNA CD Indexes (24 Indexes, 24 Samples)

20018707

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Nextera™ DNA CD Indexes (96 Indexes, 96 Samples)

20018708

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Services

5524澳门24小时线路 DNA Prep Training

20022900

Accessory Products
What accessories do I need?

5524澳门24小时线路 Purification Bead, 100mL

20060057

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5524澳门24小时线路 Purification Bead, 400mL

20060058

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Flex Lysis Reagent Kit (96 reactions)

20018706

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5524澳门24小时线路® Free Adapter Blocking Reagent (12 Reactions)

20024144

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5524澳门24小时线路® Free Adapter Blocking Reagent (48 Reactions)

20024145

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NextSeq PhiX Control Kit

FC-110-3002

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5524澳门24小时线路 DNA Prep

Product Highlights

5524澳门24小时线路 DNA Prep offers flexibility for many whole-genome sequencing applications.

  • Fastest 5524澳门24小时线路 library prep workflow, with ~3.5 hours total time
  • Flexibility to accommodate variations in sample type, DNA input amount, and application
  • Optimized library prep performance, generating reliable results

Learn more about the technology

Save Time and Resources

5524澳门24小时线路 DNA Prep uses a fast, user-friendly workflow. On-bead tagmentation chemistry reduces total library prep time to ~3.5 hours, from DNA extraction to library normalization.

Simplify Lab Operations

The 5524澳门24小时线路 DNA Prep workflow supports a broad DNA input range (1–500 ng), multiple sample types, and both small and large genomes. The workflow includes DNA extraction from blood, saliva, or dried blood spots*or bacterial colonies*.

Gain the flexibility to sequence human or other large, complex genomes as well as amplicons or microbial species, all with a single kit.

Obtain Reliable Results

While accommodating various study requirements, the 5524澳门24小时线路 DNA Prep workflow delivers consistent insert sizes, uniform coverage, and optimized performance, regardless of DNA input amount or genome size. The bead-based technology minimizes bias and opportunities for error, resulting in highly reproducible sequencing data.

Access Flexible Throughput Options

The IDT for 5524澳门24小时线路 - DNA/RNA UD Indexes Sets A, B, C, and D offer up to 384 unique dual indexes, enabling accurate assignment of reads and efficient use of the flow cell. (Sets A and B are available now. Sets C and D are coming soon.) These unique dual index codes use 10 base pair codes. This change in base pair index codes require adjustments to the sequencing run setup.

Nextera DNA CD Indexes support up to 96-sample combinatorial dual indexing. The 24 CD Indexes are supplied in a tube format, and 96 in a plate format.

Related Notification

For whole-genome sequencing, 5524澳门24小时线路 DNA Prep is the recommended replacement for the Nextera DNA Library Prep Kit, which has been discontinued. If you were using Nextera DNA (Cat. No. FC-121-1030) or stand-alone components (Cat. Nos. 15027865 and 15027866) for ATAC-Seq** or other custom applications, the 5524澳门24小时线路 Tagment DNA TDE1 Enzyme and Buffer Kit is our suggested alternative.

*Demonstrated protocols available.

**Customer-reported application

Explore 5524澳门24小时线路 Library Prep

Discover the power that 5524澳门24小时线路 library prep tagmentation technology can bring to your lab through this interactive experience. Our library prep solutions offer whole-genome sequencing across various applications, flexibility across 5524澳门24小时线路 sequencers, and integrated sample input for blood and saliva without quantification.

View Infographic
Introduction to Nextera DNA Flex Library Prep Kit

Frequently Purchased Together

Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
NextSeq 550 System 1 sample per run (high output; based on 30× coverage of a human genome) Up to 2 × 150 bp
NovaSeq 6000 System 2–10 samples per run  (dual flow cell; based on 30× coverage of a human genome) Up to 2 × 125 bp (rapid run) 
Up to 2 × 150 bp (high output)

Product Comparison

5524澳门24小时线路 DNA Prep TruSeq DNA PCR-Free TruSeq DNA Nano
Assay Time ~3-4 hours (from DNA extraction to normalized library) 5 hours total assay time ~6 hours total assay time
Automation Capability Liquid Handling Robots Liquid Handling Robots Liquid Handling Robots
Description A fast, flexible workflow for a wide range of research applications and sample types, from human to microbial whole-genome sequencing and more. A simple, all-inclusive PCR-free prep for whole-genome sequencing studies with the ability to sequence through challenging regions of the genome. A low-input research method that delivers high genome coverage quality and reduced bias.
Hands-On Time 1-1.5 hours 4 hours ~4 hours
Input Quantity Small genomes (e.g. microbial): 1-500 ng DNA. Large genomes (e.g. human): 100-500 ng DNA. (For blood and saliva, see the reference guide). 1 ug DNA 100 ng genomic DNA
Mechanism of Action Bead-linked transposome Mechanical DNA fragmentation and adapter ligation. Workflow is PCR-free and gel-free. Mechanical DNA fragmentation and adapter ligation. Workflow uses reduced-bias PCR and is gel-free.
Method Amplicon Sequencing, De Novo Sequencing, Shotgun Sequencing, Whole-Genome Sequencing Genotyping by Sequencing, Shotgun Sequencing, Whole-Genome Sequencing Genotyping by Sequencing, Shotgun Sequencing, Whole-Genome Sequencing
Multiplexing Up to 384 unique dual (UD) combinations and 96 combinatorial dual (CD) combinations Up to 24 single, 96 combinatorial (CD) dual, 24 unique dual and 96 unique dual (UD) combinations (available soon) Up to 24 single, 96 combinatorial (CD) dual, 24 unique dual and 96 unique dual (UD) combinations (available soon)
Specialized Sample Types Blood, Not FFPE-Validated, Saliva Not FFPE-Compatible Low-Input Samples, Not FFPE-Compatible
Species Category Any Species, Bacteria, Drosophila, Human, Mammalian, Mouse, Nematode, Plant, Rat, Virus, Yeast, Zebrafish Human, Mammalian, Mouse, Other, Plant, Rat Human, Mammalian, Mouse, Other, Plant, Rat
Species Details Compatible with any species Compatible with most large DNA genomes. Compatible with most large DNA genomes.
Target Insert Size ~350bp 350 bp or 550 bp 350 bp or 550 bp

Method-Specific Workflow Example

 
Prepare Library for:
Whole-Genome Sequencing
 
Sequence Efficiently with:
iSeq 100 System
MiniSeq System
MiSeq System
NextSeq 550 System
NextSeq 2000 System
NovaSeq 6000 System
 
Analyze Your Data with:
Basespace Whole-Genome Sequencing App
Variant Calling Assessment Tool
BaseSpace Sequence Hub
 

Supporting Data and Figures

5524澳门24小时线路 Bead-Linked Transposome Chemistry
5524澳门24小时线路 Bead-Linked Transposome Chemistry

(A) Bead-linked transposomes mediate the simultaneous fragmentation of gDNA and the addition of 5524澳门24小时线路 sequencing primers. (B) Reduced-cycle PCR amplification amplifies sequencing ready DNA fragments and adds indexes and adapters. (C) Sequencing-ready fragments are washed and pooled.

How It Works

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Product Literature

High Resolution Microbiome Profiling Using Whole-Metagenome Sequencing and CosmosID Cloud-Based Bioinformatics

Application Note | PDF 3 MB

An automated Nextera DNA Flex library preparation workflow for high-throughput metagenomics

Application Note | PDF < 1 MB

Nextera Crude Lysate Protocol for Metagenomic Whole-Genome Sequencing Studies

Application Note | PDF < 1 MB

Human Whole-Genome Sequencing with the Nextera DNA Flex Library Preparation Kit

Application Note | PDF < 1 MB

Direct Bacterial Colony Sequencing with the Nextera™ DNA Flex Library Preparation Kit

Application Note | PDF 1 MB

Microbial Whole-Genome Sequencing with the Nextera DNA Flex Library Preparation Kit

Application Note | PDF < 1 MB

Fast, Flexible, and Self-Normalizing Library Prep with Nextera Flex Chemistry

Application Note | HTML

Nextera DNA Flex Library Preparation for Soil Shotgun Metagenomics Analysis

Application Note | HTML

Data Sheet | PDF | 5 versions

Data Sheet | PDF | 5 versions

5524澳门24小时线路 library preparation solutions

Brochure | PDF 2 MB

Manuals and Support Information

Trim Adapters for Nextera DNA Flex in Local Run Manager for MiniSeq Documentation

5524澳门24小时线路 DNA Prep Checklist Documentation

5524澳门24小时线路 DNA Prep Support Documentation

5524澳门24小时线路 DNA Prep Reference Guide Documentation

5524澳门24小时线路 DNA Prep Consumables & Equipment List Documentation

Nextera DNA Flex Dried Blood Spot Protocol Guide Documentation

Index Adapters Pooling Guide Documentation

Custom Protocol Selector
Generates customized, end-to-end instructions

All 5524澳门24小时线路 DNA Prep  Support  

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For Research Use Only

Not for use in diagnostic procedures (except as specifically noted).

Innovative technologies

At 5524澳门24小时线路, our goal is to apply innovative technologies to the analysis of genetic variation and function, making studies possible that were not even imaginable just a few years ago. It is mission critical for us to deliver innovative, flexible, and scalable solutions to meet the needs of our customers. As a global company that places high value on collaborative interactions, rapid delivery of solutions, and providing the highest level of quality, we strive to meet this challenge. 5524澳门24小时线路 innovative sequencing and array technologies are fueling groundbreaking advancements in life science research, translational and consumer genomics, and molecular diagnostics.

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