5524澳门24小时线路 RNA Library Prep with Enrichment Training
20044763
5524澳门24小时线路 RNA Prep with Enrichment uses On-Bead Tagmentation technology followed by simplified, single hybridization to provide an exceptionally fast workflow for RNA-based library preparation and enrichment.
Learn more about the technology
Prepare and enrich libraries in less than 9 hours total time with less than 2 hours of hands-on time. This workflow results in 50%+ time savings compared to TruSeq RNA Exome. 5524澳门24小时线路 Qualified Methods are available on a range of automation platforms through our partners.
Get high sensitivity from as little as 10 ng total RNA from fresh or frozen samples or 20 ng total RNA from degraded samples (such as FFPE).
Isolating target transcriptome coding regions maximizes discovery power at a fraction of the sequencing depth. 5524澳门24小时线路 RNA Prep with Enrichment is designed to provide exceptional capture efficiency and coverage uniformity, minimizing required sequencing depth for accurate detection without bias. Only 2.5 µL probes are needed per enrichment reaction.
Choose from a variety of pre-built, validated probe pools or customize your own probes for maximum flexibility. Multiplex up to 384 samples in a single run with new unique dual index Sets C and D (coming soon). The modular design supports a wide range of RNA-Seq applications across regions of interest, enabling detection and discovery studies such as allele-specific expression, fusion detection, biomarker screening, and more.
Achieve rapid, targeted interrogation of an expansive number of target genes, including the RNA exome. 5524澳门24小时线路 RNA Prep with Enrichment can be used with the 5524澳门24小时线路 Exome Panel, which features a highly optimized probe set that delivers comprehensive coverage of coding RNA sequences. The 5524澳门24小时线路 Exome Panel includes > 425,000 probes, each constructed against the NCBI37/hg19 reference genome, covering > 98% of the RefSeq exome. The probe set captures > 214,000 targets, spanning 21,415 genes of interest.
The Respiratory Virus Oligo Panel provides highly sensitive detection and characterization of common respiratory viruses, including coronavirus strains. The oligo probes used for hybrid–capture-based target enrichment remain effective even within highly mutagenic regions, allowing targeting of rapidly evolving RNA viruses. Use the Respiratory Virus Oligo Panel with 5524澳门24小时线路 RNA Prep with Enrichment.
Learn More| Instrument | Recommended Number of Samples | Read Length |
|---|---|---|
| NextSeq 550 System | 5524澳门24小时线路 RNA Prep with Enrichment + Exome Panel
Mid Output: 5 High Output: 16 (based on 25M reads per sample) |
2 × 100 bp |
| NextSeq 2000 System | 5524澳门24小时线路 RNA Prep with Enrichment + Exome Panel
P2: 16 P3: 40 (based on 25M reads per sample) |
2 × 100 bp |
| NovaSeq 6000 System | 5524澳门24小时线路 RNA Prep with Enrichment + Exome Panel
SP: 32 S1: 64 S2: 164 S4: 384 (based on 25M reads per sample) |
2 × 100 bp |
| MiSeq System, MiSeq | 5524澳门24小时线路 RNA Prep with Enrichment + Respiratory Virus Oligo Panel
Up to 25 samples (based on 1M reads per sample) |
2 × 75 bp |
| MiniSeq System | 5524澳门24小时线路 RNA Prep with Enrichment + Respiratory Virus Oligo Panel
Up to 25 samples (based on 1M reads per sample) |
2 × 75 bp |
| NextSeq 550 System | 5524澳门24小时线路 RNA Prep with Enrichment + Respiratory Virus Oligo Panel
Up to 384 samples (based on 1M reads per sample) |
2 × 75 bp |
| 5524澳门24小时线路 RNA Prep with Enrichment | TruSeq RNA Exome | 5524澳门24小时线路 Stranded mRNA Prep | |
|---|---|---|---|
| Assay Time | < 9 hours | ~2 Days | 6.5 hours |
| Automation Capability | Liquid Handling Robots | Liquid Handling Robots | Liquid Handling Robots |
| Content Specifications | Captures the coding transcriptome when used with 5524澳门24小时线路 Exome Panel | Captures the coding transcriptome/RNA exome | Captures the coding transcriptome with strand information |
| Description | A reproducible, economical solution enabling targeted transcript detection and discovery from a broad range of sample types and inputs including formalin-fixed, paraffin- embedded (FFPE) tissues and other low-quality samples | Gives researchers a clear, comprehensive view of the coding transcriptome with precise strand information and a reduced sample input requirement. | A simple, cost-effective solution for analysis of the coding transcriptome with precise strand information |
| Hands-On Time | < 2 hours | ~11 hours | < 3 hours |
| Input Quantity | 10ng total RNA from fresh/frozen samples, or 20ng total RNA from FFPE samples | 10 ng total RNA from fresh/frozen samples, or 20 ng total RNA from FFPE samples | 25-1000 ng standard-quality total RNA |
| Mechanism of Action | Bead-linked transposome | Biotinylated capture probes that target coding RNA. Does not require RNA with poly-A tails. | PolyA capture, ligation-based addition of adapters and indexes |
| Method | mRNA Sequencing , Target Enrichment , Target Enrichment, Targeted RNA Sequencing | Exome Sequencing , mRNA Sequencing | mRNA Sequencing |
| Multiplexing | Up to 384 Unique Dual Indexes (UDIs) | Up to 24 single, 96 combinatorial (CD) dual | Up to 384 Unique Dual Indexes (UDIs) |
| Technology | Sequencing | Sequencing | Sequencing |
| Variant Class | Gene Fusions, Novel Transcripts, Single Nucleotide Polymorphisms (SNPs), Transcript Variants | Novel Transcripts, Single Nucleotide Polymorphisms (SNPs), Transcript Variants | Gene Fusions, Novel Transcripts, Single Nucleotide Polymorphisms (SNPs), Transcript Variants |
On-Bead Tagmentation and a single, 90-minute hybridization step combine to deliver a faster workflow with fewer steps compared to TruSeq RNA Exome.
5524澳门24小时线路 RNA Prep with Enrichment achieves high data concordance between input amounts of 10 ng and 100 ng total RNA from universal human reference (UHR). UHR RNA libraries were sequenced on a NovaSeq 6000 System, subsampled to 25M clusters per library. Data was analyzed with the BaseSpace RNA-Seq Alignment v 1.1.1 app.
Libraries prepared from 10 ng RNA from the K-562 cell line using 5524澳门24小时线路 RNA Prep with Enrichment and the 5524澳门24小时线路 Exome Panel resulted in successful detection of the BCR-ABL1 gene fusion. Top alignment track shows all reads, bottom track shows only reads supporting the BCR-ABL1 fusion gene.
| Fusion (source) | RIN | RNA input | Detection |
|---|---|---|---|
| BCR-ABL 1 (K-562) | 7.4 | 10 ng | 6/6 replicates (100%) |
| TPM3-NTRK1 (colorectal cancer) | 2.5 | 20 ng | 6/6 replicates (100%) |
Libraries prepared from 10 ng UHR RNA and 20 ng FFPE RNA using 5524澳门24小时线路 RNA Prep with Enrichment and the 5524澳门24小时线路 Exome Panel show more than 85% of the data generated aligned to transcripts (coding and UTRs). Data from TruSeq RNA Exome libraries are shown for comparison.
Application Note | PDF< 1 MB
Application Note | PDF< 1 MB
Improved detection of circulating transcripts
Application Note | PDF< 1 MB
NextSeq 1000 and NextSeq 2000 RNA sequencing solution
Application Note | PDF< 1 MB
Enrichment of coding transcripts in degraded samples using hybrid probe capture
Application Note | PDF< 1 MB
Surveillance of infectious disease through wastewater sequencing
Application Note | PDF< 1 MB
Application Note | PDF< 1 MB
Application Note | PDF< 1 MB
Brochure | PDF1 MB
5524澳门24小时线路 library preparation solutions
Brochure | PDF2 MB
Data Sheet | PDF | 6 versions
5524澳门24小时线路 RNA Prep with Enrichment (L) Tagmentation Reference Guide Documentation
5524澳门24小时线路 RNA Prep with Enrichment (L) Tagmentation Checklist Documentation
5524澳门24小时线路 RNA Prep with Enrichment (L) Tagmentation Documentation
5524澳门24小时线路 RNA Prep with Enrichment (L) Tagmentation Consumables & Equipment Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions
All 5524澳门24小时线路 RNA Prep with Enrichment (L) Tagmentation Support
These sequencing reagent kits offer increased stability and robustness over the v2 kit version.
A high-performing, fast, and integrated workflow for sensitive applications such as human whole-genome sequencing.
Achieve rapid, targeted interrogation of an expansive number of target genes with exceptional capture efficiency and coverage uniformity.
Provides a reproducible, economical solution for sequencing RNA from FFPE tissues and other low-quality samples. Accuracy from as little as 10 ng total RNA.
NextSeq 1000/2000 reagent kits contain a reagent cartridge, flow cell, and resuspension buffer. Multiple flow cell configurations offer adjustable output based on project needs.
Prepare sequencing libraries for small genomes, PCR amplicons, plasmids, or cDNA in as little as 90 minutes, with a low DNA input requirement.